CDC25B

Protein-coding gene in the species Homo sapiens
CDC25B
Available structures
PDBOrtholog search: PDBe RCSB
List of PDB id codes

1CWR, 1CWS, 1CWT, 1QB0, 1YM9, 1YMD, 1YMK, 1YML, 1YS0, 2A2K, 2IFD, 2IFV, 2UZQ, 3FQT, 3FQU, 4WH7, 4WH9

Identifiers
AliasesCDC25B, cell division cycle 25B
External IDsOMIM: 116949; MGI: 99701; HomoloGene: 41451; GeneCards: CDC25B; OMA:CDC25B - orthologs
Gene location (Human)
Chromosome 20 (human)
Chr.Chromosome 20 (human)[1]
Chromosome 20 (human)
Genomic location for CDC25B
Genomic location for CDC25B
Band20p13Start3,786,772 bp[1]
End3,806,121 bp[1]
Gene location (Mouse)
Chromosome 2 (mouse)
Chr.Chromosome 2 (mouse)[2]
Chromosome 2 (mouse)
Genomic location for CDC25B
Genomic location for CDC25B
Band2 F1|2 63.29 cMStart131,028,869 bp[2]
End131,040,417 bp[2]
RNA expression pattern
Bgee
HumanMouse (ortholog)
Top expressed in
  • granulocyte

  • right lung

  • right hemisphere of cerebellum

  • gastric mucosa

  • upper lobe of left lung

  • oocyte

  • stromal cell of endometrium

  • secondary oocyte

  • lymph node

  • anterior pituitary
Top expressed in
  • fetal liver hematopoietic progenitor cell

  • blood

  • tibiofemoral joint

  • ventricular zone

  • lip

  • human fetus

  • spleen

  • thymus

  • granulocyte

  • zygote
More reference expression data
BioGPS
More reference expression data
Gene ontology
Molecular function
  • protein binding
  • protein tyrosine phosphatase activity
  • hydrolase activity
  • protein kinase binding
  • phosphoprotein phosphatase activity
Cellular component
  • cytosol
  • centrosome
  • spindle pole
  • intracellular anatomical structure
  • microtubule organizing center
  • cytoskeleton
  • nucleus
  • nucleoplasm
  • cytoplasm
Biological process
  • protein dephosphorylation
  • positive regulation of mitotic cell cycle
  • cell division
  • positive regulation of cytokinesis
  • protein phosphorylation
  • G2/M transition of mitotic cell cycle
  • oocyte maturation
  • positive regulation of cell population proliferation
  • cell cycle
  • female meiosis I
  • positive regulation of protein kinase activity
  • peptidyl-tyrosine dephosphorylation
  • positive regulation of cell cycle G2/M phase transition
  • mitotic cell cycle
  • positive regulation of G2/M transition of mitotic cell cycle
  • positive regulation of G2/MI transition of meiotic cell cycle
Sources:Amigo / QuickGO
Orthologs
SpeciesHumanMouse
Entrez

994

12531

Ensembl

ENSG00000101224

ENSMUSG00000027330

UniProt

P30305

P30306

RefSeq (mRNA)
NM_001287516
NM_001287517
NM_001287518
NM_001287519
NM_001287520

NM_001287522
NM_001287524
NM_004358
NM_021872
NM_021873
NM_021874
NM_212530

NM_001111075
NM_023117

RefSeq (protein)
NP_001274445
NP_001274446
NP_001274447
NP_001274448
NP_001274449

NP_001274451
NP_001274453
NP_004349
NP_068658
NP_068659

NP_001104545
NP_075606

Location (UCSC)Chr 20: 3.79 – 3.81 MbChr 2: 131.03 – 131.04 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

M-phase inducer phosphatase 2 is an enzyme that in humans is encoded by the CDC25B gene.[5]

CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.[6]

Interactions

CDC25B has been shown to interact with MAPK14,[7] Casein kinase 2, alpha 1,[8] CHEK1,[9] MELK,[10] Estrogen receptor alpha,[11] YWHAB,[12][13] YWHAZ,[12] YWHAH[12] and YWHAE.[12][13]

References

  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000101224 – Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000027330 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ Galaktionov K, Beach D (February 1992). "Specific activation of cdc25 tyrosine phosphatases by B-type cyclins: evidence for multiple roles of mitotic cyclins". Cell. 67 (6): 1181–94. doi:10.1016/0092-8674(91)90294-9. PMID 1836978. S2CID 9659637.
  6. ^ "Entrez Gene: CDC25B cell division cycle 25 homolog B (S. pombe)".
  7. ^ Bulavin DV, Higashimoto Y, Popoff I J, Gaarde W A, Basrur V, Potapova O, Appella E, Fornace A J (May 2001). "Initiation of a G2/M checkpoint after ultraviolet radiation requires p38 kinase". Nature. 411 (6833): 102–7. doi:10.1038/35075107. ISSN 0028-0836. PMID 11333986. S2CID 4410763.
  8. ^ Theis-Febvre N, Filhol Odile, Froment Carine, Cazales Martine, Cochet Claude, Monsarrat Bernard, Ducommun Bernard, Baldin Véronique (January 2003). "Protein kinase CK2 regulates CDC25B phosphatase activity". Oncogene. 22 (2): 220–32. doi:10.1038/sj.onc.1206107. ISSN 0950-9232. PMID 12527891. S2CID 11296989.
  9. ^ Sanchez Y, Wong C, Thoma R S, Richman R, Wu Z, Piwnica-Worms H, Elledge S J (September 1997). "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25". Science. 277 (5331): 1497–501. doi:10.1126/science.277.5331.1497. ISSN 0036-8075. PMID 9278511.
  10. ^ Davezac N, Baldin Véronique, Blot Joëlle, Ducommun Bernard, Tassan Jean-Pierre (October 2002). "Human pEg3 kinase associates with and phosphorylates CDC25B phosphatase: a potential role for pEg3 in cell cycle regulation". Oncogene. 21 (50): 7630–41. doi:10.1038/sj.onc.1205870. ISSN 0950-9232. PMID 12400006. S2CID 20678893.
  11. ^ Ma ZQ, Liu Z, Ngan E S, Tsai S Y (December 2001). "Cdc25B Functions as a Novel Coactivator for the Steroid Receptors". Mol. Cell. Biol. 21 (23): 8056–67. doi:10.1128/MCB.21.23.8056-8067.2001. ISSN 0270-7306. PMC 99972. PMID 11689696.
  12. ^ a b c d Mils V, Baldin V, Goubin F, Pinta I, Papin C, Waye M, Eychene A, Ducommun B (March 2000). "Specific interaction between 14-3-3 isoforms and the human CDC25B phosphatase". Oncogene. 19 (10): 1257–65. doi:10.1038/sj.onc.1203419. ISSN 0950-9232. PMID 10713667. S2CID 5816838.
  13. ^ a b Conklin DS, Galaktionov K, Beach D (August 1995). "14-3-3 proteins associate with cdc25 phosphatases". Proc. Natl. Acad. Sci. U.S.A. 92 (17): 7892–6. Bibcode:1995PNAS...92.7892C. doi:10.1073/pnas.92.17.7892. ISSN 0027-8424. PMC 41252. PMID 7644510.

Further reading

  • Draetta G, Eckstein J (1997). "Cdc25 protein phosphatases in cell proliferation". Biochim. Biophys. Acta. 1332 (2): M53–63. doi:10.1016/S0304-419X(96)00049-2. PMID 9141461.
  • Nilsson I, Hoffmann I (2000). "Cell cycle regulation by the Cdc25 phosphatase family". Progress in Cell Cycle Research. Vol. 4. pp. 107–14. doi:10.1007/978-1-4615-4253-7_10. ISBN 978-1-4613-6909-7. PMID 10740819.
  • Nagata A, Igarashi M, Jinno S, et al. (1992). "An additional homolog of the fission yeast cdc25+ gene occurs in humans and is highly expressed in some cancer cells". New Biol. 3 (10): 959–68. PMID 1662986.
  • Conklin DS, Galaktionov K, Beach D (1995). "14-3-3 proteins associate with cdc25 phosphatases". Proc. Natl. Acad. Sci. U.S.A. 92 (17): 7892–6. Bibcode:1995PNAS...92.7892C. doi:10.1073/pnas.92.17.7892. PMC 41252. PMID 7644510.
  • Galaktionov K, Lee AK, Eckstein J, et al. (1995). "CDC25 phosphatases as potential human oncogenes". Science. 269 (5230): 1575–7. Bibcode:1995Sci...269.1575G. doi:10.1126/science.7667636. PMID 7667636.
  • Demetrick DJ, Beach DH (1994). "Chromosome mapping of human CDC25A and CDC25B phosphatases". Genomics. 18 (1): 144–7. doi:10.1006/geno.1993.1440. PMID 8276402.
  • Honda R, Ohba Y, Nagata A, et al. (1993). "Dephosphorylation of human p34cdc2 kinase on both Thr-14 and Tyr-15 by human cdc25B phosphatase". FEBS Lett. 318 (3): 331–4. doi:10.1016/0014-5793(93)80540-B. PMID 8440392. S2CID 30754963.
  • Lane SA, Baker E, Sutherland GR, et al. (1993). "The human cell cycle gene CDC25B is located at 20p13". Genomics. 15 (3): 693–4. doi:10.1006/geno.1993.1129. PMID 8468065.
  • Baldin V, Cans C, Superti-Furga G, Ducommun B (1997). "Alternative splicing of the human CDC25B tyrosine phosphatase. Possible implications for growth control?". Oncogene. 14 (20): 2485–95. doi:10.1038/sj.onc.1201063. PMID 9188863. S2CID 6164215.
  • Booher RN, Holman PS, Fattaey A (1997). "Human Myt1 is a cell cycle-regulated kinase that inhibits Cdc2 but not Cdk2 activity". J. Biol. Chem. 272 (35): 22300–6. doi:10.1074/jbc.272.35.22300. PMID 9268380.
  • Sanchez Y, Wong C, Thoma RS, et al. (1997). "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25". Science. 277 (5331): 1497–501. doi:10.1126/science.277.5331.1497. PMID 9278511.
  • Jin P, Hardy S, Morgan DO (1998). "Nuclear Localization of Cyclin B1 Controls Mitotic Entry After DNA Damage". J. Cell Biol. 141 (4): 875–85. doi:10.1083/jcb.141.4.875. PMC 2132764. PMID 9585407.
  • Hofmann K, Bucher P, Kajava AV (1998). "A model of Cdc25 phosphatase catalytic domain and Cdk-interaction surface based on the presence of a rhodanese homology domain". J. Mol. Biol. 282 (1): 195–208. doi:10.1006/jmbi.1998.1998. PMID 9733650.
  • Forrest AR, McCormack AK, DeSouza CP, et al. (1999). "Multiple splicing variants of cdc25B regulate G2/M progression". Biochem. Biophys. Res. Commun. 260 (2): 510–5. doi:10.1006/bbrc.1999.0870. PMID 10403798.
  • Karlsson C, Katich S, Hagting A, et al. (1999). "Cdc25b and Cdc25c Differ Markedly in Their Properties as Initiators of Mitosis". J. Cell Biol. 146 (3): 573–84. doi:10.1083/jcb.146.3.573. PMC 2150562. PMID 10444066.
  • Reynolds RA, Yem AW, Wolfe CL, et al. (1999). "Crystal structure of the catalytic subunit of Cdc25B required for G2/M phase transition of the cell cycle". J. Mol. Biol. 293 (3): 559–68. doi:10.1006/jmbi.1999.3168. PMID 10543950.
  • Mils V, Baldin V, Goubin F, et al. (2000). "Specific interaction between 14-3-3 isoforms and the human CDC25B phosphatase". Oncogene. 19 (10): 1257–65. doi:10.1038/sj.onc.1203419. PMID 10713667. S2CID 5816838.
  • v
  • t
  • e
  • 1cwr: HUMAN CDC25B CATALYTIC DOMAIN WITHOUT ION IN CATALYTIC SITE
    1cwr: HUMAN CDC25B CATALYTIC DOMAIN WITHOUT ION IN CATALYTIC SITE
  • 1cws: HUMAN CDC25B CATALYTIC DOMAIN WITH TUNGSTATE
    1cws: HUMAN CDC25B CATALYTIC DOMAIN WITH TUNGSTATE
  • 1cwt: HUMAN CDC25B CATALYTIC DOMAIN WITH METHYL MERCURY
    1cwt: HUMAN CDC25B CATALYTIC DOMAIN WITH METHYL MERCURY
  • 1qb0: HUMAN CDC25B CATALYTIC DOMAIN
    1qb0: HUMAN CDC25B CATALYTIC DOMAIN
  • 1ym9: Crystal structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the sulfinic form
    1ym9: Crystal structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the sulfinic form
  • 1ymd: Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the sulfonic form
    1ymd: Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the sulfonic form
  • 1ymk: Crystal Structure of the CDC25B phosphatase catalytic domain in the apo form
    1ymk: Crystal Structure of the CDC25B phosphatase catalytic domain in the apo form
  • 1yml: Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the sulfenic form
    1yml: Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the sulfenic form
  • 1ys0: Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the disulfide form
    1ys0: Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the disulfide form
  • 2a2k: Crystal Structure of an active site mutant, C473S, of Cdc25B Phosphatase Catalytic Domain
    2a2k: Crystal Structure of an active site mutant, C473S, of Cdc25B Phosphatase Catalytic Domain
  • 2ifd: Crystal structure of a remote binding site mutant, R492L, of CDC25B Phosphatase catalytic domain
    2ifd: Crystal structure of a remote binding site mutant, R492L, of CDC25B Phosphatase catalytic domain
  • 2ifv: Crystal structure of an active site mutant, C473D, of CDC25B phosphatase catalytic domain
    2ifv: Crystal structure of an active site mutant, C473D, of CDC25B phosphatase catalytic domain
  • v
  • t
  • e
Class I
Classical PTPs
Receptor type PTPs
Non receptor type PTPs
VH1-like or
dual specific
phosphatases
(DSPs)
MAPK phosphatases (MKPs)
Slingshots
PRLs
CDC14s
Atypical DSPs
Phosphatase and tensin
homologs (PTENs)
Myotubularins
Class II
Class III
Class IV

External links