Rank | Heat | Lane | Athlete | Result | Notes |
1 | 1 | 4 | João Gomes Jr. (BRA) | 28.21 | Q |
2 | 2 | 4 | Felipe França Silva (BRA) | 28.29 | Q |
3 | 1 | 3 | Martin Melconian (URU) | 28.91 | Q |
4 | 3 | 5 | Genaro Britez (PAR) | 29.02 | Q |
5 | 1 | 5 | Lucas Eugenio Peralta (ARG) | 29.19 | Q |
6 | 3 | 6 | Renato Fernandez (PAR) | 29.58 | Q |
7 | 2 | 5 | Gonzalo Martin Acuna (ARG) | 29.59 | Q |
7 | 3 | 3 | Jorge Murillo (COL) | 29.59 | Q |
9 | 2 | 3 | Jefersson Llanos (COL) | 29.75 | |
10 | 3 | 2 | Alex Solorzano Castro (ECU) | 29.77 | |
11 | 2 | 2 | Ricardo Fuentes Salgado (CHI) | 30.77 | |
12 | 1 | 2 | Hycinth Cijntje (AHO) | 30.80 | |
13 | 3 | 7 | Jose Salazar Sierra (ECU) | 30.94 | |
14 | 2 | 6 | Pedro Luna Llamosas (PER) | 31.50 | |
15 | 1 | 6 | Erick Arturo Medina (PAN) | 31.55 | |
16 | 2 | 7 | Diguan Pigot (SUR) | 31.58 | |
17 | 3 | 1 | Jordy Groters (ARU) | 32.08 | |
18 | 1 | 7 | Mario Sergio Chicot (PER) | 33.28 | |
19 | 1 | 1 | Fabian Binns (GUY) | 34.08 | |
20 | 2 | 1 | Armando Claure (BOL) | 35.62 | |
| 3 | 4 | Ivan Enderica Ochoa (ECU) | DNS | |